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Equipment and consumables

We house the following equipment in the lab: 

  • a mosquito LCP pipetting robot
  • robotics facilities for large-scale screening and production of 'de novo' optimisation screens
  • facilities for manual setup, as well as crystal manipulation and freezing
  • two temperature-controlled rooms: one at 4°c and the other at 20°c (this one has automatic crystal storage and imaging systems for crystal detection)
  • three RUMED incubators at 10°c, 16°c and one for custom temperature setup
  • a facility for in-house data collection using a modern rigaku detector with CCD image collection equipment. 

EQUIPMENT BY LOCATION 

X-RAY ROOM (B16F)  

  • Generator (Rigaku Micromax -007 HF) 
  • Optics (Varimax HF Optics) 
  • Cryosystem (700 series) 
  • Detector (Rigaku Saturn 944 CCD detector) 

ROBOTICS LABORATORY (B16A)  

  • Deep Well Block Transfer (Liquidator) 
  • Nanoliter pipetting (Mosquito) 
  • Plate databasing and barcoding (CrystalTrak) 
  • Screen optimisation (Multiprobe II) 

20°C TEMPERATURE CONTROLLED ROOM  

  • Rigaku Desktop Minstrel: Imaging and protein crystal monitoring using automated UV microscopy 
  • Nikon SMZ-2B Stereoscopic Microscope with polariser  

4°C TEMPERATURE CONTROLLED ROOM  

  • Nikon SMZ-2B Stereoscope Microscope with polariser
  • Microcentrifuge 

MAIN LABORATORY  

  • Leica M165 microscope with LCD display for crystal acquisition
  • Microcentrifuge
  • Plate centrifuge
  • Micro scales
  • General scales
  • pH meter
  • Stirrers and hot plate
  • Small filter pump
  • Sonicator
  • Vortex 

LABORATORY CONSUMABLES  

SCREENS

  • Hampton: Index, SaltRx, MembFac, Crystal Lite 
  • Molecular Dimensions: Structure, JCSG, Pro Plex, PACT, PGA, Memstart, MemGold, MemSys, Memplus, MIDAS, Morpheus I-III 
  • Qiagen: pH clear, NH4SO4, MPD, MbCLass, PEG's
  • Additive Screens: Detergent, Additive, SilverBullet, SilverBullet Bio, MemAdvantage

STOCK CHEMICALS

  • Poly-ethylene Glycols: 400, 1500, 2000, 3000, 3350, 4000, 6000, 8000, 550mme, 5000mme
  • Solvents: Ethanol, Methanol, Isopropanol
  • Salts: NH4SO4, NaCl, NaSO4, LiCl, LiSO4, MgCl2, KCl, KBr, KNa Tartrate, Na Tartrate, Na Acetate, NH4 Citrate, Na Malonate, Na Formate, Mg Formate, ZnCl2
  • Buffers: Glycine, Lactate, Acetate, Citrate, Bicine, Tris, Bis-Tris, HEPES, Bis-Tris Propane, MES, MOPS, CAPS, CHES
  • HCl, NaOH
  • Cryo protectants: MPD, Ethylene glycol, Glycerol, Sorbitol, Sucrose, CryoProtX

GENERAL CONSUMABLES

  • 1 and 2 ml 96-Well plates
  • 0.5 and 1.5ml tubes
  • 15 and 50 ml falcon tubes
  • 10k MWCO 0.5ml concentrators
  • 0.22 μM stock/sample filtration
  • 100 μL buffer exchange
  • 96-well Swissci 2 and 3 well plates
  • 24-well Limbro and 48 well VWX plates (siliconized)
  • LCP plate set up