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Equipment

The ISMB Electron Microscopy Facility enables a wide range of sample preparation and imaging experiments with containment level 2. Training prior to use is mandatory and all equipment should be booked in advance.

Sample Preparation

  • Vitrobot IV
  • Leica GP2
  • Manual plunge freezer
  • Leica HPM-100 High Pressure Freezer
  • Freeze substitution
  • Aquilos 2+ (including cryo lift-out)
  • Room temperature and cryo microtomes

Electron Microscopes

  • Tecnai 10
  • Tecnai 12
  • Tecnai F20
  • Krios G3i with K3 direct electron detector and BioQuantum energy filter, Falcon 3 detector, phase plate, FFI

CLEM

  • Leica Thunder
  • Integrated Fluorescence Light Microscope (iFLM) on Aquilos 2