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EMBO practical course 2013

We are pleased to announce the EMBO practical course on image processing for cryo-electron microscopy. This 10 day course will run from 3 September – 13 September 2013 at Birkbeck, Malet Street, London WC1E 7HX, United Kingdom.

The EMBO course is supported by EMBO, and also by FEI, Direct Electron, JEOL, Gatan, and LEICA.

Objectives of the course

Structural analysis by cryo EM has become a major tool for studying large macromolecular complexes. The use of field emission gun microscopes, imaging of samples at liquid nitrogen temperatures, and developments in image processing have led to improvements in the resolution of single particle structures from 20-30 Å to 6-10 Å. More complex processing is required to extract the higher resolution details. Cryo EM single particle analysis is an ideal complement to X-ray crystallography and NMR. Fitting of individual structures into cryo EM maps of large assemblies allows the characterization of multi-component interactions, to distinguish different functional states. This hybrid approach is essential for understanding biological mechanisms and is an important addition to structural genomics. New approaches developed for analysis of thin cells and cryo-sections by tomography extend the range of EM from individual complexes to cellular structures in situ.

The aim of the course is to teach the basic principles and practical aspects of image processing to bioscientists and structural biologists wishing to determine macromolecular structures by cryo electron microscopy (EM). The course will concentrate on processing of single particle images, and will be aimed at advanced PhD students and postdocs using cryo EM images for structural analysis.

Course organisers

The course is organised by Professor Elena Orlova and Professor Helen Saibil from the Department of Biological Sciences, Birkbeck.