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EMBO Practical Course 2015

EMBO Practical Course on Image Processing for Cryo-electron Microscopy
Date: date  – date 2015
Location:
Birkbeck, Malet Street, London WC1E 7HX, United Kingdom.

The EMBO course is supported by EMBO, and also by FEI, Direct Electron, JEOL, Gatan, and LEICA.

Objectives of the course

  • The aim of the course is to teach the basic principles and practical aspects of image processing to bioscientists and structural biologists who wish to determine macromolecular structures by cryo electron microscopy (EM).
  • The course will concentrate on processing of single particle images and will be aimed at advanced PhD students and postdocs using cryo EM images for structural analysis.

Structural analysis by cryo EM has become a major tool for studying large macromolecular complexes. The use of field emission gun microscopes, imaging of samples at liquid nitrogen temperatures, and developments in image processing have led to improvements in the resolution of single particle structures from 20-30 Å to 6-10 Å. More complex processing is required to extract the higher resolution details.

Cryo EM single particle analysis is an ideal complement to X-ray crystallography and NMR. Fitting of individual structures into cryo EM maps of large assemblies allows the characterization of multi-component interactions to distinguish different functional states. This hybrid approach is essential for understanding biological mechanisms and is an important addition to structural genomics. New approaches developed for analysis of thin cells and cryo-sections by tomography extend the range of EM from individual complexes to cellular structures in situ.

Course content details

Course organisers

The course is organised by Professor Elena Orlova and Professor Helen Saibil from the Department of Biological Sciences, Birkbeck.

Lecturers

  • The following people will be speaking at the course:
    • name link (link to new dept profiles where appropriate)
    • name link2

Fee details

  • There will be a x EUR registration fee for accepted participants (except for participants from industry who will be charged a fee of x EUR).
    The registration fee includes all food and lodging for the duration of the course, but all attendees must arrange and pay for their own travel.

How to apply

  • Applications are invited from PhD students and researchers in EM. Applications should include:
    • A single PDF or Word file containing a CV, a publication list, a short description of the applicant's current work and future plans and an explanation of why attendance at the course would further the applicant's research.
    • A letter of recommendation from the applicant's supervisor (to be sent by the supervisor)
    Applications should be sent by email to: Bryony Stewart-Seume (b.stewart-seume@bbk.ac.uk) Applicants will be contacted two or three weeks after the deadline to confirm whether their application has been successful.

Accommodation and arrival details

  • The course will be held at Birkbeck College in central London and accommodation will be provided nearby on the nights of date - date 2015. Further details of accommodation and instructions for arrival will be sent to all course attendees.