Dr Jerome Gouge
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Research
Research
Research interests
- Oxidative stress response
- Transcription
- Structural Biology
Research overview
Reactive oxygen species (ROS) are highly reactive molecules produced by cells during fundamental processes (e.g. metabolism, respiration…) or after exposure to external factors (e.g. pollution). Although ROS play an important role in many cell functions (e.g. differentiation), high levels of ROS can damage cells and alter genetic information. Therefore, cells need to control ROS levels and ensure that they remain at a healthy level. This is called maintaining the ‘redox balance’. Problems in maintaining this balance are a cause and a consequence of several human diseases, including cancer and neurodegenerative diseases. Drugs that maintain the redox balance may help to treat ROS-related diseases.
Despite their importance in human health and disease, the mechanisms underlying the redox balance remain poorly understood. We are interested in understanding how cells detect and respond to changes to the redox balance, and how these responses are deregulated, especially in cancer. We are using Structural Biology (cryo-EM and crystallography), Biophysics and Biochemistry as core techniques in the laboratory.Research Centres and Institutes
Post doctoral staff
- Tabitha Jenkins
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Supervision and teaching
Supervision and teaching
Teaching
Teaching modules
- Advanced Cell Biology (BCBC006S6)
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Publications
Publications
Article
- Kun Yu, B.Y. and Tossounian, M.-A. and Denchev Hristov, S. and Lawrence, R. and Arora, P. and Tsuchiya, Y. and Peak-Chew, S.Y. and Filonenko, V. and Oxenford, S. and Angell, R. and Gouge, Jerome and Skehel, M. and Goutac, M. (2021) Regulation of metastasis suppressor NME1 by a key metabolic cofactor coenzyme A. Redox Biology 44 (101978), ISSN 2213-2317.
- Jenkins, Tabi and Gouge, Jerome (2021) Nrf2 in cancer, detoxifying enzymes and cell death programs. Antioxidants 10 (7), ISSN 2076-3921.
- Ramsay, E.P. and Abascal-Palacios, G. and Daiß, J.L. and King, H. and Gouge, Jerome and Pilsl, M. and Beuron, F. and Morris, E. and Gunkel, P. and Engel, C. and Vannini, A. (2020) Structure of human RNA polymerase III. Nature Communications 11 (6409), ISSN 2041-1723.
- Sachdeva, A. and Gouge, Jerome and Kontovounisios, C. and Nikolaou, S. and Ashworth, A. and Lim, K. and Chong, I. (2020) Klotho and the treatment of human malignancies. Cancers 12 (1665), ISSN 2072-6694.
- Kramm, K. and Schroder, T. and Gouge, Jerome and Vera, A.M. and Gupta, K. and Heiss, F.B. and Engel, C. and Berger, I. and Vannini, A. and Tinnefeld, P. and Grohmann, D. (2020) DNA origami-based single-molecule forcespectroscopy elucidates RNA Polymerase IIIpre-initiation complex stability. Nature Communications 11 (2828), ISSN 2041-1723.
- Dergai, O. and Cousin, P. and Gouge, Jerome and Satia, K. and Praz, V. and Kuhlman, T. and Lhôte, P. and Vannini, A. and Hernandez, N. (2018) Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. Genes & Development 32 (9-10), pp. 711-722. ISSN 1549-5477.
- Gouge, Jerome and Vannini, A. (2017) New tricks for an old dog: Brf2-dependent RNA Polymerase III transcription in oxidative stress and cancer. Transcription 9 (1), pp. 61-66. ISSN 2154-1272.
- Gouge, Jerome and Guthertz, N. and Kramm, K. and Dergai, O. and Abascal-Palacios, G. and Satia, K. and Cousin, P. and Hernandez, N. and Grohmann, D. and Vannini, A. (2017) Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation. Nature Communications 8 (1), pp. 130. ISSN 2041-1723.
- Gouge, Jerome and Satia, K. and Guthertz, N. and Widya, M. and Thompson, A.J. and Cousin, P. and Dergai, O. and Hernandez, N. and Vannini, A. (2015) Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.. Cell 163 (6), pp. 1375-87. ISSN 1097-4172.
- Gouge, Jerome and Rosario, S. and Romain, F. and Poitevin, F. and Béguin, P. and Delarue, M. (2015) Structural basis for a novel mechanism of DNA bridging and alignment in eukaryotic DSB DNA repair. The EMBO Journal 34 (8), pp. 1126-42. ISSN 0261-4189.
- Gouge, Jerome and Rosario, S. and Romain, F. and Beguin, P. and Delarue, M. (2013) Structures of intermediates along the catalytic cycle of terminal deoxynucleotidyltransferase: dynamical aspects of the two-metal ion mechanism. Journal of Molecular Biology 425 (22), pp. 4334-52. ISSN 0022-2836.
- Costi, R. and Crucitti, G.C. and Pescatori, L. and Messore, A. and Scipione, L. and Tortorella, S. and Amoroso, A. and Crespan, E. and Campiglia, P. and Maresca, B. and Porta, A. and Granata, I. and Novellino, E. and Gouge, Jerome and Delarue, M. and Maga, G. and Di Santo, R. (2013) New nucleotide-competitive non-nucleoside inhibitors of terminal deoxynucleotidyl transferase: discovery, characterization, and crystal structure in complex with the target. Journal of Medicinal Chemistry 56 (18), pp. 7431-41. ISSN 0022-2623.
- Gouge, Jerome and Ralec, C. and Henneke, G. and Delarue, M. (2012) Molecular recognition of canonical and deaminated bases by P. abyssi family B DNA polymerase. Journal of Molecular Biology 423 (3), pp. 315-36. ISSN 0022-2836.
- Romain, F. and Barbosa, I. and Gouge, Jerome and Rougeon, F. and Delarue, M. (2009) Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region. Nucleic Acids Research 37 (14), pp. 4642-56. ISSN 0305-1048.