Prof Elena Orlova

Positions held:

Professor of Macromolecular Systems, Department of Biological Sciences

Supervision and teaching
Supervision and teaching

Teaching

Teaching modules
- Structural Bioinformatics (CRYS005H7)
- Macromolecular and Cellular Electron Microscopy (SCBS061S7)
Publications
Publications
Article
- Ignatiou, I. and Brasilès, S. and El Sadek Fadel, M. and Bürger, J. and Mielke, T. and Topf, Maya and Tavares, P. and Orlova, Elena (2019) Structural transitions during the scaffolding-driven assembly of a viral capsid. Nature Communications 10, pp. 4840. ISSN 2041-1723.
- Ameh, E.M. and Tyrrel, S. and Harris, J. and Ignatiou, A. and Orlova, Elena and Nocker, A. (2018) The absence or presence of a lytic coliphage affects the response of Escherichia coli to heat, chlorine, or UV exposure. Folia Microbiologica 63 (5), pp. 599-606. ISSN 0015-5632.
- Redzej, Adam and Ukleja, Marta and Connery, Sarah and Trokter, Martina and Felisberto‐Rodrigues, Catarina and Cryar, A. and Thalassinos, Konstantinos and Hayward, Richard D. and Orlova, Elena V. and Waksman, Gabriel (2017) Structure of a VirD4 coupling protein bound to a VirB type IV secretion machinery. The EMBO Journal 36 (19), pp. e201796629. ISSN 0261-4189.
- Gordon, J.E. and Costa, Tiago and Patel, R.S. and Gonzalez-Rivera, C. and Sarkar, M.K. and Orlova, Elena and Waksman, Gabriel and Christie, P.J. (2017) Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation. Molecular Microbiology 105 (2), pp. 273-293. ISSN 0950-382X.
- Cuniasse, P. and Tavares, P. and Orlova, Elena and Zinn-Justin, S. (2017) Structures of biomolecular complexes by combination of NMR and cryoEM methods. Current Opinion in Structural Biology 43, pp. 104-113. ISSN 0959-440X.
- White, Hayley and Ignatiou, Athenasios and Clare, Daniel and Orlova, Elena (2017) Structural study of heterogeneous biological samples by Cryoelectron Microscopy and image processing. BioMed Research International pp. 1032432. ISSN 2314-6133.
- Costa, Tiago and Ignatiou, Athenasios and Orlova, Elena (2017) Structural analysis of protein complexes by cryo electron microscopy. Methods in Molecular Biology 1615, pp. 377-413. ISSN 1064-3745.
- Javed, A. and Christodoulou, John and Cabrita, L.D. and Orlova, Elena (2017) The ribosome and its role in protein folding: looking through a magnifying glass. Acta Crystallographica Section D Biological Crystallography 73 (6), pp. 509-521. ISSN 0907-4449.
- Kearns, Sinead and Lurz, R. and Orlova, Elena and Okorokov, A.L. (2016) Two p53 tetramers bind one consensus DNA response element. Nucleic Acids Research 44 (13), pp. 6185-6199. ISSN 0305-1048.
- Clare, Daniel Kofi and Pechnikova, E.V. and Skurat, E.V. and Makarov, V.V. and Sokolova, O.S. and Solovyev, A.G. and Orlova, Elena (2015) Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by Cryo-Electron Microscopy. Structure 23 (10), pp. 1815-1826. ISSN 0969-2126.
- Chaban, Yuriy and Stead, J.A. and Ryzhenkova, Ksenia and Whelan, F. and Lamber, Katerina and Antson, A. and Sanders, C.M. and Orlova, Elena (2015) Structural basis for DNA strand separation by a hexameric replicative helicase. Nucleic Acids Research 43 (17), pp. 8551-8563. ISSN 0305-1048.
- Chaban, Yuriy and Lurz, R. and Brasilès, S. and Cornilleau, C. and Karreman, Matthia and Zinn-Justin, S. and Tavares, P. and Orlova, Elena (2015) Structural rearrangements in the phage head-to-tail interface during assembly and infection. Proceedings of the National Academy of Sciences of the United States of America 112 (22), pp. 7009-7014. ISSN 0027-8424.
- Orlova, Elena (2015) A molecular syringe that kills cells. Nature Structural & Molecular Biology 22 (5), pp. 357-359. ISSN 1545-9993.
- Langlois, C. and Ramboarina, S. and Cukkemane, A. and Auzat, I. and Chagot, B. and Gilquin, B. and Ignatiou, A. and Petitpas, I. and Kasotakis, E. and Paternostre, M. and White, H. E. and Orlova, Elena and Baldus, M. and Tavares, P. and Zinn-Justin, S. (2015) Bacteriophage SPP1 tail tube protein self-assembles into β-structure rich tubes. Journal of Biological Chemistry 390, pp. 3836-3849. ISSN 0021-9258.
- Hesketh, E.L. and Parker-Manuel, R.P. and Chaban, Y. and Satti, R. and Coverley, D. and Orlova, Elena and Chong, James P.J. (2015) DNA induces conformational changes in a recombinant human minichromosome maintenance complex. Journal of Biological Chemistry pp. jbc.M114.622738. ISSN 0021-9258.
- Bestembayeva, A. and Kramer, A. and Labokha, A.A. and Osmanović, D. and Liashkovich, I. and Orlova, Elena and Ford, I.J. and Charras, G. and Fassati, A. and Hoogenboom, B.W. (2015) Nanoscale stiffness topography reveals structure and mechanics of the transport barrier in intact nuclear pore complexes. Nature Nanotechnology 10 (1), pp. 60-64. ISSN 1748-3387.
- Redzej, Adam and Waksman, Gabriel and Orlova, Elena (2015) Structural studies of T4S systems by electron microscopy. AIMS Biophysics 2 (2), pp. 184-199. ISSN 2377-9098.
- Low, Harry H. and Gubellini, Francesca and Rivera-Calzada, Angel and Braun, Nathalie and Connery, Sarah and Dujeancourt, A. and Lu, Fang and Redzej, Adam and Fronzes, R. and Orlova, Elena and Waksman, Gabriel (2014) Structure of a type IV secretion system. Nature 508, pp. 550-553. ISSN 0028-0836.
- Waksman, Gabriel and Orlova, Elena (2014) Structural organisation of the type IV secretion systems. Current Opinion in Microbiology 17, pp. 24-31. ISSN 1369-5274.
- Rivera-Calzada, A. and Fronzes, Remi and Savva, Christos G. and Chandran, Vidya and Lian, Pei W. and Laeremans, T. and Pardon, E. and Steyaert, J. and Remaut, Han and Waksman, Gabriel and Orlova, Elena (2013) Structure of a bacterial type IV secretion core complex at subnanometre resolution. The EMBO Journal 32 (8), pp. 1195-1204. ISSN 1460-4189.
- Milanesi, Lilia and Sheynis, T. and Xue, W.-F. and Orlova, Elena and Hellewell, A.L. and Jelinek, R. and Hewitt, E.W. and Radford, S.E. and Saibil, Helen R. (2012) Direct three-dimensional visualization of membrane disruption by amyloid fibrils. Proceedings of the National Academy of Sciences of the United States of America 109 (50), pp. 20455-20460. ISSN 0027-8424.
- Walldén, Karin and Williams, Robert and Yan, Jun and Lian, Pei W. and Wang, Luchun and Thalassinos, Konstantinos and Orlova, Elena and Waksman, Gabriel (2012) Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system. Proceedings of the National Academy of Sciences of the United States of America 109 (28), pp. 11348-11353. ISSN 0027-8424.
- White, Helen E. and Sherman, M.B. and Brasiles, S. and Jacquet, E. and Seavers, P. and Tavares, P. and Orlova, Elena (2012) Capsid structure and its stability at the late stages of bacteriophage SPP1 assembly. Journal of Virology 86 (12), pp. 6768-6777. ISSN 0022-538X.
- Aramayo, Ricardo and Sherman, M.B. and Brownless, K. and Lurz, R. and Okorokov, A.L. and Orlova, Elena (2011) Quaternary structure of the specific p53-DNA complex reveals the mechanism of p53 mutant dominance. Nucleic Acids Research 39 (20), pp. 8960-8971. ISSN 0305-1048.
- Orlova, Elena and Saibil, Helen R. (2011) Structural analysis of macromolecular assemblies by electron microscopy. Chemical Reviews 111 (12), pp. 7710-7748. ISSN 0009-2665.
- Law, R.H.P. and Lukoyanova, Natalya and Voskoboinik, I. and Caradoc-Davies, T.T. and Baran, K. and Dunstone, M.A. and d’Angelo, M.E. and Orlova, Elena and Coulibaly, F. and Verschoor, S. and Browne, K.A. and Ciccone, A. and Kuiper, M.J. and Bird, P.I. and Trapani, J.A. and Saibil, Helen R. and Whisstock, J.C. (2010) The structural basis for membrane binding and pore formation by lymphocyte perforin. Nature 468 (7322), pp. 447-451. ISSN 0028-0836.
- Clare, Daniel K. and Orlova, Elena (2010) 4.6Å Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300keV on a 4k×4k CCD camera. Journal of Structural Biology 171 (3), pp. 303-308. ISSN 1047-8477.
- Okorokov, A.L. and Chaban, Y.L. and Bugreev, D.V. and Hodgkinson, J.L. and Mazin, A.V. and Orlova, Elena (2010) Structure of the hDmc1-ssDNA filament reveals the principles of its architecture. PLoS One 5 (1), ISSN 1932-6203.
- Clare, Daniel K. and Orlova, Elena (2010) 4.6 Å cryo-em reconstruction of tobacco mosaic virus from images recorded at 300 keV on a 4k × 4k CCD camera. Journal of Structural Biology 171 (3), pp. 303-308. ISSN 1047-8477.
- Lhuillier, S. and Gallopin, M. and Gilquin, B. and Brasiles, S. and Lancelot, N. and Letellier, G. and Gilles, M. and Dethan, G. and Orlova, Elena and Couprie, J. and Tavares, P. and Zinn-Justin, S. (2009) Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating. Proceedings of the National Academy of Sciences of the United States of America 106 (21), pp. 8507-8512. ISSN 0027-8424.
- White, Helen E. and Hodgkinson, J.L. and Jahn, T.R. and Cohen-Krausz, Sara and Gosal, W.S. and Muller, S. and Orlova, Elena and Radford, S.E. and Saibil, Helen R. (2009) Globular Tetramers of beta(2)-Microglobulin assemble into elaborate amyloid fibrils. Journal of Molecular Biology 389 (1), pp. 48-57. ISSN 0022-2836.
- Okorokov, A.L. and Orlova, Elena (2009) Structural biology of the p53 tumour suppressor. Current Opinion in Structural Biology 19 (2), pp. 197-202. ISSN 0959-440X.
- Orlova, Elena (2009) How viruses infect bacteria?. Embo Journal 28, pp. 797-798. ISSN 0261-4189.
- Fronzes, Remi and Schafer, Eva and Wang, Luchun and Saibil, Helen R. and Orlova, Elena and Waksman, Gabriel (2009) Structure of a type IV secretion system core complex. Science 323 (5911), pp. 266-268. ISSN 0036-8075.
- Salih, O. and Remaut, Han and Waksman, Gabriel and Orlova, Elena (2008) Structural analysis of the Saf pilus by electron microscopy and image processing. Journal of Molecular Biology 379 (1), pp. 174-187. ISSN 0022-2836.
- Elad, Nadav and Clare, Daniel K. and Saibil, Helen R. and Orlova, Elena (2008) Detection and separation of heterogeneity in molecular complexes by statistical analysis of their two-dimensional projections. Journal of Structural Biology 162 (1), pp. 108-120. ISSN 1047-8477.
- Elad, Nadav and Farr, G.W. and Clare, Daniel K. and Orlova, Elena and Horwich, A.L. and Saibil, Helen R. (2007) Topologies of a Substrate Protein Bound to the Chaperonin GroEL. Molecular Cell 26 (3), pp. 415 - 426. ISSN 1097-2765.
- Tattum, M.H. and Cohen-Krausz, Sara and Khalili Shirazi, A. and Jackson, G.S. and Orlova, Elena and Collinge, J. and Clarke, A.R. and Saibil, Helen R. (2006) Elongated oligomers assemble into mammalian PrP Amyloid fibrils. Journal of Molecular Biology 357 (3), pp. 975-985. ISSN 0022-2836.
Book Section
- Javed, A. and Orlova, Elena (2020) Unravelling Ribosome function through structural studies. In: Harris, J.R. and Marles-Wright, J. (eds.) Macromolecular Protein Complexes II: Structure and Function. Subcellular Biochemistry. Springer. ISBN 9783030281502. (In Press)
- White, H.E. and Orlova, Elena (2019) Bacteriophages: their structural organisation and function. In: Bacteriophages - Biology and Applications [Working Title]. IntechOpen.
- Coscia, F. and Ryzhenkova, K. and Chaban, Yuriy and Sanders, C. and Orlova, Elena (2016) Single particle EM study of the E1 helicase from Papillomavirus in complex with DNA. In: European Microscopy Congress 2016 Proceedings. Wiley-VCH Verlag GmbH & Co. KGaA. pp. 37-38. ISBN 9783527342976.
- Ryzhenkova, K. and Coscia, F. and Chaban, Yuriy and Sanders, C. and Orlova, Elena (2016) Structural study of the BPV E1 helicase/DNA complex using electron microscopy. In: European Microscopy Congress 2016 Proceedings. Wiley-VCH Verlag GmbH & Co. KGaA. pp. 39-40. ISBN 9783527342976.
- Orlova, Elena (2012) Bacteriophages and their structural organisation. In: Kurtboke, I. (ed.) Bacteriophages. Rijeka, Croatia: InTech. pp. 3-30. ISBN 9789535102724.
- Tavares, P. and Zinn-Justin, S. and Orlova, Elena (2011) Genome gating in tailed bacteriophage capsids. In: Rossmann, M.G. and Rao, V.B. (eds.) Viral Molecular Machines. Advances in Experimental Medicine and Biology. New York, U.S.: Springer. pp. 585-600. ISBN 9781461409809.
- Orlova, Elena and Saibil, Helen R. (2010) Methods for three-dimensional reconstruction of heterogeneous assemblies. In: Jensen, G.J. (ed.) Methods in Enzymology. Cryo-EM, Part B: 3-D Reconstruction. Philadelphia, USA: Elsevier. pp. 321-341. ISBN 9780123849915.

Prof Elena Orlova

Supervision and teaching

Teaching

Teaching modules

Publications

Article

Book Section