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Dr Anastasia Mylona

Birkbeck Career Development ISSF Fellow and Principal Investigator

Contact details

email: a.mylona@mail.cryst.bbk.ac.uk
tel: +44 (0) 20 7631 6553
office: 324b
lab:320

Institute of Structural & Molecular Biology (ISMB), Department of Biological Sciences, Birkbeck, Malet Street, London, WC1E 7HX

 

Research interests

  • Protein phosphorylation is an important post-translational protein modification and a major regulatory mechanism of cellular signaling.  Deregulation of phosphorylation pathways commonly underlies disease aetiology. Proteins often get phosphorylated not only at one but at multiple sites by a single protein kinase. Multi-site phosphorylation is a key regulatory mechanism in many cellular processes, and has been suggested to provide a precise tool to generate complex functional outputs from single kinase signaling inputs.

    Our research vision is to understand the mechanism and functional role of multi-site phosphorylation in physiology and disease. In particular, we want to understand how multisite phosphorylation controls the activity of transcriptional regulators and subsequently the final biological response and how it can alter the architecture of signaling pathways in diseases like cancer, which may pave the way for novel treatments. For that purpose, we use a novel multidisciplinary methodology pipeline combining structural (time-resolved NMR, X-ray crystallography), biochemical and cell-based in vivo experimental strategies.

Selected publications

  • Mylona A, Carr S, Allen P, Moraes I, Treisman R, Evans G and Foadi J. A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software. Crystals 2017, 7(8), 242; doi:10.3390/cryst7080242

    Mylona A, Theillet X-F, Foster C, Cheng T, Miralles F, Selenko P, and Treisman R. Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation. Science 2016 Oct 14; 354(6309): 233-237.

    As featured in: Whitmarsh AJ & Davis RJ. Multisite phosphorylation by MAPK. Science 2016 Oct 14; 354(6309): 179-180.

    Chakraborty A, Diefenbacher ME, Mylona A, Kassel O, and Behrens A. The E3 ubiquitin ligase TRIM7 mediates c-Jun/AP-1 activation by Ras signalling. Nat Commun. 2015 Apr 8;6:6782.

    Mylona A, Nicolas R, Maurice D, Sargent M, Tuil D, Daegelen D, Treisman R, and Costello P. The essential function for serum response factor in T-cell development reflects its specific coupling to extracellular signal-regulated kinase signaling. Mol Cell Biol. 2011 Jan;31(2):267-76.

    Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, and Müller C.W. Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly. Mol Cell. 2006 Oct 20;24(2):221-32.

Teaching/PhD supervision

  • We are always looking for talented students to join the lab. Please contact me to discuss possibilities and projects.

Dr Anastasia Mylona