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Methods in Integrative Structural Biology

For MRes and PhD students

X-ray crystallograpy, cryoEM, spectroscopies and biophysical characterisations

This lecture course on structural biology is especially for MRes and PhD students. MRes Structural Biology students and first year full-time PhD students should attend, and all other members of the Department are also invited.

Sessions take place on Thursdays and include discussion and question sessions, as well as practicals with equipment and computer software.

The course is organised by Dr Tracey Barrett

2020/21 timetable

All sessions take place on Thursdays from 09:30-11:00. Speakers will be presenting remotely via Microsoft Teams; an invitation link will be sent out for each session.

Lecture handouts

Click the title of the talk below to access the handouts for each lecture (handouts will be made available shortly before each week's session). You will need to use the login details provided by email to access the file.

Autumn Term 2020

Date/time

Title/s

Speaker/s

Location

Thursday 8 October 2020

09:30-11:00

Diffraction theory, basis of crystallography and EM

Professor Helen Saibil

Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 15 October 2020

09:30-11:00

Protein expression and purification

Dr Claire Bagneris

Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 22 October 2020

09:30-11:00

Crystallisation and Crystallographic data collection

Dr TC Tan and Dr Nikos Pinotsis

PC room or Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 29 October 2020

No lecture

No lecture

 

Thursday 5 November 2020


09:30-11:00

Structure determination – Molecular replacement, Experimental phasing

Dr Tracey Barrett

PC room or Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 12 November 2020


09:30-11:00

Crystallography refinement and model building

Professor Nicholas Keep and Dr Nikos Pinotsis

PC room or Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 19 November 2020


09:30-11:00

EM sample preparation and data collection

Professor Carolyn Moores and Dr Natasha Lukoyanova

Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 26 November 2020


09:30-11:00

Image formation, contrast, CTF

Professor Helen Saibil

Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 3 December 2020


09:30-11:00

Alignment and classification; 3D reconstruction and validation

Professor Elena Orlova

Remotely via Microsoft Teams; an invitation link will be sent out

Thursday 10 December 2020


09:30-11:00

TBC

TBC

Remotely via Microsoft Teams; an invitation link will be sent out

Spring Term 2021

Date

Title

Speaker(s)

Location

Thursday 14 January 2021


09:30-11:00

Characterisation of protein samples and complexes by biophysical methods

Dr Nikos Pinotsis

B62/3

or online

Thursday 21 January 2021


09:30-11:00

Protein structure and interactions by NMR

Dr Mark Williams

B62/3

or online

Thursday 28 January 2021


09:30-11:00

NMR: Protein dynamics

Professor Flemming Hansen

B62/3

or online

Thursday 4 February 2021


09:30-11:00

Single molecule methods: TIRF, FRET, AFM, optical trapping

Dr Anthony Roberts

B62/3

or online

Thursday 11 February  2021


09:30-11:00

Next Generation Sequencing

Dr Irilenia Nobeli

B62/3

or online

Thursday 18  February 2021

No lecture

No Lecture

Thursday 25 February 2021

09:30-11:00

Combining native, ion mobility and crosslinking mass spectrometry to study protein structure and dynamics

Professor Kostas Thalassinos

B62/3

or online

Thursday 4 March 2021


09:30-11:00

CD & IR spectroscopy

Professor Bonnie Wallace and Dr Amandine Maréchal

B62/3

or online

Thursday 11 March 2021

09:30-11:00

Protein-protein docking, integrative modelling and model validation

Professor Maya Topf

B62/3

or online

Thursday 18 March 2021

No Lecture

No lecture